STEP ONE

TECHNICAL PRODUCT BULLETIN #B-43
USEPA, OIL PROGRAM CENTER
ORIGINAL LISTING DATE: MARCH 12, 1992
REVISED LISTING DATE: MARCH 21, 1997
"STEP ONE"
(a/k/a B&S INDUSTRIAL)

I. NAME, BRAND, OR TRADEMARK
STEP ONE
Type of Product: Biological Additive
II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
B & S Research, Inc.
4345 Highway 21
Embarrass, MN 55732
Phone: (218) 984-3757
Fax: (218) 984-3212
Email: farmforprofit@frontier.com
(Mr. H.W. Lashmett)
III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
B&S Research, Inc.
4345 Highway 21
Embarrass, MN 55732
Phone: (218) 984-3757
Fax: (218) 984-3212
Email: farmforprofit@frontier.com
(Mr. H.W. Lashmett)

Bioremediation International
4345 Highway 21
Embarrass, MN 55732
Phone: (218) 984-3757
Fax: (218) 984-3212
Email: farmforprofit@frontier.com

Farm for Profit Research
and Development
4345 Highway 21
Embarrass, MN 55732
Phone: (800) 232-7692
Fax: (218) 984-3212
Email: farmforprofit@frontier.com
IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability:
None
2. Ventilation:
Normal ventilation is adequate
3. Skin and eye contact; protective clothing; treatment in case of contact:
Avoid contact with skin, especially when there are open wounds. In case of skin contact, wash with soapy water. The use of protective gloves is recommended. Avoid contact with eyes. In case of eye contact, immediately flush eyes with plenty of water continuously for at least 15 minutes. Consult a physician. The use of protective goggles is recommended. Avoid inhalation and ingestion. It is recommended that workers wear dust mask and not eat or smoke while handling the product(s).
4.a. Maximum storage temperature: 90ºF
4.b. Minimum storage temperature: -30ºF
4.c. Optimum storage temperature range: 32ºF to 70ºF
4.d. Temperature phase separations and chemical changes: NA
V. SHELF LIFE
Over 3 years
VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method:
Components of the product STEP ONE (BC101) and STEP ONE (MSE2.5) are only sold together. B&S Research will make application recommendations based on contamination and ppm of contamination at time of purchase order and employ specified application methods (spray, plowing, agitation, etc.) appropriate for a particular situation.
2. Concentration/Application Rate:
Nominally, the STEP ONE water mixture can be used on most hydrocarbons, including crude and refined petroleum products, pesticides, etc., on land, fresh water or ocean water. Laboratory tests also indicate effectiveness in destruction of PCB's. In certain circumstances, B&S Research, Inc. may require laboratory soil and water contamination tests before the product may be used (see VI 1. above).
VII. TOXICITY AND EFFECTIVENESS
a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:
DAYS PRODUCT
3 REPS/PROD
TOTAL MEAN
ALKANES (ppm)
RED%
28 DAYS
TOTAL MEAN
AROMATICS (ppm)
RED%
28 DAYS
0 CONTROL
NUTRIENT
STEP ONE
29959.5
29008.5
30477.8

 
5391.8
5163.0
5499.8

 
7 CONTROL
NUTRIENT
STEP ONE
33471.3
22723.7
24196.8

 
5720.9
5050.1
3110.2

 
28 CONTROL
NUTRIENT
STEP ONE
30997.4
1103.9
17059.4
0.0 %
96.19%
44.03%
5388.9
4582.4
2501.9
0.0 %
11.23%
54.51%


Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 19%
Product (STEP ONE): 51%
VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6-8
Temperature: 70ºF to 90ºF
Salinity: Fresh or salt (ocean < 110 ppm) water, land or dry surfaces
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: < 5 or > 9, (apply neutralizing agents -- lime or P205)
Temperature: < 50ºF or > 135ºF
Salinity: < 0 or > 110 ppm
4. Special nutrient requirements:
None
5. Test results regarding the determination of the presence of the following:
Salmonella: None found
Fecal coliform: None found
Shigella: None found
Staphylococcus coagulase positive: None found
Beta hemolytic Streptococci: None found
IX. PHYSICAL PROPERTIES
1. Flash Point: > 212ºF
2. Pour Point: -7ºF
3. Viscosity:
2.8 cst at 50ºF
3 5 cst at 32ºF
5.9 cst at -4ºF
4. Specific Gravity: 1.03 at 59ºF
5. pH: 7.2 (1:2.5:80 BC101:MSE2.5:water mixture)
6. Chemical Name and Percentage by Weight of the Total Formulation:
CONFIDENTIAL
7. Surface Active Agents: Noedal
8. Solvents: None
9. Additives: Phosphoric acid as P205
3% emulsifier < .5% by weight
10. Solubility: Infinite
X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
Compound Concentration (ppm)
Arsenic < 0.0200
Cadmium < 0.0030
Chromium 0.0859
Copper 0.0177
Lead 0.0090
Mercury 0.0002
Nickel < 0.0080
Zinc 0.0602
Cyanide 0.2830
Chlorinated Hydrocarbons < 0.0050

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