WMI-2000

TECHNICAL PRODUCT BULLETIN #B-19
USEPA, OIL PROGRAM CENTER
ORIGINAL LISTING DATE: JUNE 18, 1990
REVISED LISTING DATE: JANUARY 11, 1996
"WMI-2000"

I. NAME, BRAND, OR TRADEMARK
WMI-2000
Type of Product: Biological Additive
II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
WMI International, Inc.
4901 Milwee Street, Suite 109
Houston, TX 77092
Phone: (713) 956-4001
Mobile: (832) 798-5610
Fax: (713) 956-7305
Email: wmiintlinc@yahoo.com
(Joseph F. Jennings, President)
III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
WMI International, Inc.
4901 Milwee Street, Suite 109
Houston, TX 77092
Phone: (713) 956-4001
Mobile: (832) 798-5610
Fax: (713) 956-7305
Email: wmiintlinc@yahoo.com
(Joseph F. Jennings, President)
IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability:
WMI-2000 is non-flammable.
2. Ventilation:
Avoid breathing dust or aerosol due to possibility of sensitization. Use adequate ventilation.
3. Skin and eye contact; protective clothing; treatment in case of contact:
Minimize contact with eyes, skin, and clothing. Wash hands thoroughly after handling culture.
4.a. Maximum storage temperature: 100ºF
4.b. Minimum storage temperature: 35ºF
4.c. Optimum storage temperature range: 45ºF - 90ºF
Store in a cool, dry location. Keep partially-used containers tightly closed. Prolonged exposure to high temperature and humidity may lower activity of product.
4.d. Temperatures of phase separations and chemical changes: NA
V. SHELF LIFE
The shelf life of WMI-2000 is 2 years if stored at ambient temperatures
VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method:
The culture should be activated for 2 hours in water, when applicable. Conditions for use vary depending on substrate concentration and type, pH, temperature, availability of nutrients, oxygen content, and liquid medium (static or flowing).
2. Concentration/Application Rate:
Inoculation concentration is 5-9 billion spores per gram.
3. Conditions for Use:
Temperature requirements are between 35ºF and 100ºF. The optimum dissolved oxygen content is 2 ppm at the sludge-to-water interface. Nitrogen and phosphorous are key nutrients which promote cultural growth, and should be maintained at concentrations of 15-20 ppm and 2.5-5 ppm, respectively. The optimum pH range is between 7.0 and 8.0.
VII. TOXICITY AND EFFECTIVENESS
a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:
DAYS PRODUCT
3 REPS/PROD
TOTAL MEAN
ALKANES (ppm)
RED%
28 DAYS
TOTAL MEAN
AROMATICS (ppm)
RED%
28 DAYS
0 CONTROL
NUTRIENT
WMI-2000
2626.7
2763.3
2453.3
0
0
0
1850.0
2030.0
1880.0
0
0
0
7 CONTROL
NUTRIENT
WMI-2000
2246.7
1920.0
1243.3
0
0
0
1823.3
1666.7
1456.7
0
0
0
28 CONTROL
NUTRIENT
WMI-2000
2240.0
1210.0
973.3
14.7
56.2
60.3
1866.7
1480.0
1253.0
-0.9
27.0
33.3


Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 25%
Product (WMI-2000): 44%
VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentage in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additives:
Optimum pH: 7.0
Optimum temperature: 45ºF - 90ºF
Optimum salinity: Fresh water to sea water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 6.0 - 8.0
Temperature: 40ºF - 120ºF
Salinity range: Fresh to sea water
4. Special nutrient requirements:
none
5. Test results regarding the determination of the following:
Salmonella - Negative
Fecal Coliform - Negative
Shigella - Negative
Staphylococcus Coagulase positive - Negative
Beta hemolytic streptococcus - Negative
IX. PHYSICAL PROPERTIES
NA
X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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